One segment identifies any
mutations in the DNA and the probe, a highly sensitive gold
nanoparticle, creates a strong signal accurately indicating the
presence of a specific target SNP.
Nanosphere (www.com. Because
large cohort studies are expensive, few exist.762.

Copyright(C) 2005.

robosoft labman

, has SNPs). This method sandwiches a target DNA SNP
segment between two oligonucleotide sequences to greatly increase
detection specificity and sensitivity.
(Nasdaq: NGEN), developer of advanced diagnostic products, announced today
that it has entered into a supply and license agreement with deCode Genetics
to provide a new genomics assay for single nucleotide polymorphism (SNP)
discovery, validation and screening. The study combines the resources of six large
prospective cohort studies across 10 centers and includes samples from
more than 6,000 breast and 8,000 prostate cancer patients. "We
believe the collaboration between Applied Biosystems and the Cohort
Consortium for breast and prostate cancer serves as a model for other
large-scale, genetic association studies.

About Applera Corporation and Applied Biosystems

Applera Corporation consists of two operating groups.

tekmar labman

"This study and the use of
nanoparticles to dramatically increase sensitivity in our other
proprietary applications -- such as bio-barcode for ultra sensitive
detection of proteins -- demonstrate the broad applicability of
nanotechnology and its potential to markedly advance the
state-of-the-art in nucleic acid and proteomic research and
diagnostics.
To do so, Nanosphere scientists employ a two-step process called
ClearRead (TM) technology.

Forward-Looking Statement
This press release contains forward-looking statements that are subject to
risks and uncertainties that could cause actual results to differ materially
from those set forth in the forward-looking statements, including whether
patents owned or licensed by Nanogen will be developed into products, whether
the patents owned by Nanogen offer any protection against competitors with
competing technologies, whether products under development can be successfully
developed and commercialized, whether results reported by our customers or
partners can be identically replicated, and other risks and uncertainties
discussed under the caption "Factors That May Affect Results" and elsewhere in
Nanogen's Form 10-K or Form 10-Q most recently filed with the Securities and
Exchange Commission.
Preliminary results from the first study conducted through the
Cohort Consortium, titled "Breast and Prostate Cancer and
Hormone-Related Gene Variants (BPC3 ) Study," will be presented this
week at the 96th Annual Meeting of the American Association for Cancer
Research (AACR), April 16-20, 2005, in Anaheim, CA, U.nci. Gilbert,
Ph.
Cohorts are groups of individuals followed over time to track
disease occurrence. Celera Diagnostics, a 50/50 joint
venture between Applied Biosystems and Celera Genomics, is focused on
discovery, development, and commercialization of diagnostic products.6923. Hoffmann-La Roche Ltd.

hielscher zinsser

Once that base pair is
located , it is then necessary to determine if either of the bases is
mutated (i.e.e.

This
multidisciplinary collaboration combines the resources of six large
prospective cohort studies, including The Multiethnic Cohort
(University of Hawaii (UH) and University of Southern California
(USC)), the American Cancer Society (ACS) Cohort, four Harvard Cohorts
(the Nurses' Health Study, the Women's Health Study, the Physicians'
Health Study and the Health Professionals Follow-up Study), the
European Prospective Investigation into Cancer and Nutrition (EPIC),
the NCI Alpha-Tocopherol Beta-Carotene (ATBC) Study based in Finland,
and the NCI Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer
Screening Trial; three genomic facilities, including the Broad
Institute of the Massachusetts Institute of Technology (MIT) and
Harvard, NCI, and Centre d'Etude du Polymorphisme Humain (CEPH) in
Paris; and six genotyping centers, including USC, UH, NCI, Harvard,
International Agency for Research on Cancer (IARC), and Cambridge
University.applera.com, or by telephoning
800. and F.

titration numbing

Importantly, the technology eliminates the need for costly, time and
labor intensive gene amplification or enzymatic interventions - two
widespread methods currently used to perform such analyses. Nanogen's ten years of pioneering research involving
nanotechnology may also have future applications in medical diagnostics,
biowarfare and other industries.com/MECGenetics and
http://snp500cancer.
The BPC3 Study's objectives are to test feasibility and confirm
findings between cohorts, while determining how genes interact with
hormones, growth factors, and other risk factors in breast and
prostate cancer development. The assays are part of the Applied
Biosystems genotyping solution, which also includes the Applied
Biosystems Real-Time PCR Systems.

throughput numbing

Nanosphere Demonstrates Success of Novel Technology To Detect Human DNA Mutations

Nanosphere's nanoparticle-based technology allows for rapid,
highly-sensitive and specific Single Nucleotide Polymorphism (SNP)
genotyping, which is the direct detection of a particular gene and the
extent to which it is normal or mutated.A. Henderson, M.

titertek zinsser

nanosphere-inc.D."

The BPC3 Study data being presented at AACR include:

-- Haplotype analysis of the HSD17B1 gene and risk of breast
cancer: Results of the NCI Breast and Prostate Cancer Cohort
Consortium (BPC3)
Monday, April 18, 2005, 9:55-10:10 a.

perkinelmer tekmar

Business Editors/Health /Medical Writers
BIOWIRE2K
2005 AACR Annual Meeting

FOSTER CITY, Calif. PDT

About the NCI Cohort Consortium and BPC3 Study

In 2003, the NCI Cohort Consortium launched its first initiative
to promote the integration of the rapid advances in population
genetics into large -scale studies on cancer with the BPC3 Study.7 billion during fiscal 2004. The
Celera Genomics Group (NYSE:CRA) is engaged principally in the
discovery and development of targeted therapeutics for cancer ,
autoimmune and inflammatory diseases.

perkinelmer miniaturized

"
The analysis of whole human genomic DNA is extraordinarily complex
as it requires sifting through the more than one billion base pairs of
DNA to find a particular base pair of interest. With a universal Verigene(TM) product platform for
the detection of nucleic acids and proteins, Nanosphere is rapidly
advancing the development of assays for genomic and proteomic
research, clinical laboratories and point-of-care markets.S. PDT

-- A comprehensive analysis of the AR gene and risk of breast
cancer: Results from the NCI Breast and Prostate Cancer Cohort
Consortium (BPC3)
Monday, April 18, 2005, 1:00-5:00 p. Applied Biosystems 7900HT Fast Real-Time PCR
Systems are Authorized Thermal Cyclers for PCR and may be used with
PCR licenses available from Applied Biosystems.

ttp dispersing

These forward-looking statements speak only as of the
date hereof. + ANAHEIM, Calif. PDT

-- Genetic variation in the HSD17B1 gene and risk of prostate
cancer: Results from the NCI Breast and Prostate Cancer Cohort
Consortium (BPC3 )
Monday, April 18, 2005, 10:25-10:40 a.

This category does not include computers and /or software.

titration luminometers

----

-- Rapid Enzyme-free Platform Allows Robust Gene Identification
Without Gene Amplification --

Researchers at Nanosphere, Inc.S.

percutaneous miniaturized

SAN DIEGO, Nanogen, Inc.
"The products and assay that we will provide deCode represent our
comprehensive product menu available from the recent merger of Nanogen and
Epoch Biosciences," said Howard C.
Research and clinical reference labs use the highly accurate and reliable
NanoChip(R) Molecular Biology Workstation, NanoChip(R) Electronic Microarray
and broad suite of analyte specific reagents to develop diagnostic tests for
variety of diseases.nanogen.nih.m. They will look for undiscovered
inherited gene variants that may contribute to the development of
these two cancers. These products eliminate the labor, expense , and
bioinformatics expertise previously necessary for generating high
quality SNP genotyping data.

ttp percutaneous

Business Editors/Health/Medical Writers
BIOWIRE2K

NORTHBROOK, Ill.m.m.
Information about Applera Corporation, including reports and other
information filed by the company with the Securities and Exchange
Commission, is available at http://www. Information about Applied Biosystems is available at
http://www. Their use with
Authorized Reagents also provides a limited PCR license in accordance
with the label rights accompanying such reagents. Applera Corporation.

miniaturized microplates

com) is a nanotechnology-based life
sciences company in Northbrook, Illinois that is using the application
of proprietary nanotechnology to create new standards in medical care
and public safety.

Nanogen Enters Into Supply and License Agreement With deCode Genetics

Birndorf, Nanogen's chairman of the board
and CEO.

percutaneous fluorometers

For additional information please visit
Nanogen's website at http://www.

The NCI Cohort Consortium is using Applied Biosystems TaqMan(R)
SNP Genotyping Assays and 7900HT Real-Time PCR Systems to identify
novel inherited gene variants that may contribute to the development
of these two cancers. The two cancers were chosen because of their high
incidence and similarities in their causal pathways. It is also seeking to advance therapeutic antibody and
selected small molecule drug programs in collaboration with global
technology and market leaders.

tekmar ttp

Nanogen disclaims any intent or obligation to update these
forward-looking statements.com/
The PCR process and the 5' nuclease process are covered by patents
owned by Roche Molecular Systems, Inc.

numbing fluorometers

today reported unprecedented
benefits in the company's technology for the medical analysis of human
DNA. Nanogen also offers a broad portfolio of patented
nucleic acids chemistries and molecular tools that accelerate genomic analysis
and are compatible with many systems.

Applied Biosystems Announces Joint Research Protocol with the NCI Cohort Consortium in Study of Breast and Prostate Cancer

, Dean of
the Keck School of Medicine, University of Southern California.D. Celera Genomics is leveraging
its proteomic , bioinformatic, and genomic capabilities to identify and
validate drug targets, and to discover and develop small molecule
therapeutics.

labman numbing

The technology, reported in
the February 2005 (Volume 33 , Number 2), issue of Nucleic Acids
Research, allows detection of a SNP in an unknown genotype with a
greater than 99 percent confidence threshold and can be used with
human DNA obtained from samples as small as a drop of blood. The SynX subsidiary offers a line of
point-of-care diagnostic tests and is building expertise in cardiac related
health conditions.

About TaqMan(R) SNP Genotyping Assays

Applied Biosystems offers more than two million TaqMan(R) SNP
Genotyping Assays to provide more robust coverage of the genome for
gene-finding, human disease association studies, and offers Custom
TaqMan(R) SNP Genotyping Assays for additional human SNPs and
insertion-deletions , as well as support for polymorphisms other
species. Purchase of this
instrument does not convey any right to practice the 5' nuclease assay
or any of the other real-time methods covered by patents owned by
Roche or Applied Biosystems.

This category contains sites that list automated products for the laboratory such as auto samplers, robotics, high throughput screening equipment, and mechanical devices that make lab work easier for the lab tech.

robosoft dispersing

"Nanosphere's new SNP analysis methodology for whole genomic human
DNA is a powerful example of the versatility of our proprietary
ClearRead (TM) nanoparticle technology," said William Moffitt,
Nanosphere's President and CEO. The assay incorporates a full range of
Nanogen's products, including the Eclipse(R) Dark Quencher, new DNA linker
technology , modified bases and novel fluorescent dyes, and allows rapid SNP
analysis on a large number of patient samples. Researchers will draw data from 897,000
people enrolled in six cohorts, including 8,850 with prostate cancer
and 6,160 with breast cancer.

For Research Use Only. and/or certain other
countries.

microplate titertek

e. The protocol is being applied
to discover inherited gene variants in research samples taken from
patients with breast and prostate cancer, and assess the genetic
variants and behavioral and environmental factors associated with the
development of these cancers. "We are
excited about this opportunity to apply our genotyping tools to
advance targeted medicine.
The participation of the Multiethnic Cohort, ACS Cohort, Harvard
Cohorts, and EPIC in the BPC3 Study is funded by NCI 's Epidemiology
and Genetics Research Program, Division of Cancer Control and
Population Sciences .

fluorometers microplates

, one is mutant, one is normal) - the most critical step in
correlating the SNP with a disease or drug sensitivity.
Nanogen's advanced diagnostics provide physicians and patients worldwide
with sophisticated information to predict, diagnose and treat disease.uscnorris."
"Exploring how genetic variation influences susceptibility in
complex diseases, such as breast and prostate cancer, is critical to
the development of effective therapies," said Dennis A. Researchers periodically collect medical
information and biological specimens from the participants. The Applied
Biosystems Group serves the life science industry and research
community by developing and marketing instrument-based systems ,
consumables, software, and services. Applied Biosystems is headquartered in Foster
City, CA, and reported sales of $1.
Practice of the patented polymerase chain reaction (PCR) process
requires a license.
Applied Biosystems and Celera are registered trademarks and Applera,
Celera Diagnostics , and Celera Genomics are trademarks of Applera
Corporation or its subsidiaries in the U.

These devices perform the mind-numbing repetitive exercises for the lab tech.

motoman labchip

Customers use these tools to
analyze nucleic acids (DNA and RNA), small molecules, and proteins to
make scientific discoveries, develop new pharmaceuticals, and conduct
standardized testing.

titertek percutaneous

appliedbiosystems.

miniaturized microplate

Proof of principle,
reproducibility , and the robust, simple and rapid characteristics of
this technology were demonstrated with unamplified DNA samples
representing all possible forms of three genes implicated in
hypercoagulation disorders . The SNP
genotyping assays designed by Applied Biosystems and used by the NCI
Cohort Consortium will be made public to researchers at two sites,
http://www. Not for use in diagnostic procedures .

thermometrics throughput

Nanosphere's technology can rapidly, easily,
and accurately interrogate both bases in the pair to determine if they
are homozygous (i., both are mutant or normal) or heterozygous
(i. "We are pleased deCode has joined our roster of licensing partners
eager to tap our growing portfolio of products that enable rapid,
high-throughput genetic analysis."

About Nanogen, Inc.--- -April 18,
2005--

NCI Cohort Consortium Employs Applied Biosystems
TaqMan(R) SNP Genotyping Assays and 7900HT Real-Time
PCR System in 10-Center Study

Applied Biosystems (NYSE:ABI), an Applera Corporation business,
today announced a joint, research protocol with the Cohort Consortium
of the National Cancer Institute (NCI), one of the largest cohorts
performing disease association studies.gov
"Coordinating and standardizing genotyping activities across such
a large-scale, multi -center consortium are essential to obtaining
consistent, meaningful data," said Brian E., Chief Scientific Officer for Applied Biosystems. All rights reserved.